Application Note: WB: 1:2000-1:10000. ELISA: 1:20000-1:100000. IHC: 1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: Pre-adsorbed with Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat serum proteins. May react with immunoglobulins from other species.
Form: Liquid
Buffer (with preservative): 20mM Potassium Phosphate, 150mM NaCl, 1% BSA, 0.01% Sodium azide.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Sheep
Immunogen: Sheep IgG whole molecule
Target Isotype: IgG
Purification: Purified by antigen-affinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
Conjugation: Biotin