Clone: R001
Specificity: Human EGF. Has cross-reactivity with Canine EGF and Mouse EGF in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human EGF / Epidermal Growth Factor protein
Gene: EGF
Description: EGF antibody LS-C818944 is an unconjugated rabbit monoclonal antibody to human EGF. Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA, 1. 2 µg/ml of this antibody will block >80% of the binding of 5 µg/ml of biotinylated Human EGF to immobilized Recombinant Human EGFR coated at 10 µg/ml (100 µL/well) in the plate. Neutralization: The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2. 5-7. 5 ug/ml in the presence of 2 ng/mL Recombinant Human EGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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