Clone: RE01
Specificity: Human EGFR. Has cross-reactivity with Rhesus EGFR in ELISA assay. No cross-reactivity with Mouse EGFR or Rat EGFR in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human EGFR / HER1 / ErbB1 protein
Gene: EGFR
Description: EGFR antibody LS-C818946 is an unconjugated rabbit monoclonal antibody to human EGFR. Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized the recombinant Human EGF at 2 ug/ml (100 µl/well), 1 ug/ml of the Human EGFR antibody can blocked > 50% the binding of recombinant receptor EGFR at 0.08 ug/ml. Neutralization: The neutralization activity of EGFR Neutralizing Antibody is Measured by its ability to neutralize autocrine EGF induced proliferation in the human breast cancer MDA-MB-468 cell line. The Neutralization titer (IC50) is typically 0.05-0.2 ug/ml for 5000 cells/well. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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