Target Synonym: EIF3I;EIF3S2;PRO2242;TRIP-1;TRIP1;eIF3-beta;eIF3-p36
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of human EIF3I
Dilution: WB 1:500-1:2000
Calculated MW: 36kDa
Observed MW: 37kDa
Deutsch
English
