Firefly Luciferase-eGFP Lentivirus (Puromycin)-79980-P

Firefly Luciferase-eGFP Lentivirus (Puromycin)-79980-P
Artikelnummer
BPS79980-P
Verpackungseinheit
500 µl x 2
Hersteller
BPS Bioscience

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Products from BPS Bioscience require a minimum order value above 400€

Application: Ideal as a positive control for transduction; useful for transduction optimization.

Description: The Firefly Luciferase-eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. Both the luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells, allowing greater flexibility for detection of transduced cells.

Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of Firefly Luciferase-eGFP lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Biosafety: of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Biosafety Level: BSL-2
Mehr Informationen
Artikelnummer BPS79980-P
Hersteller BPS Bioscience
Hersteller Artikelnummer 79980-P
Verpackungseinheit 500 µl x 2
Mengeneinheit STK
Produktinformation (PDF) Download
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