Usage: This ELISA kit applies to the in vitro quantitative determination of FMN concentrations in ..
Tested Sample Types: serum, plasma and other biological fluids
Type: Competitive-ELISA
Assay Time: 2 h 30 min
Detection Type: Colormetric
Sensitivity: 9.38 pg/mL
Detection Range: 15.63-1000 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal FMN. During the reaction, Universal FMN in the sample or standard competes with a fixed amount of Universal FMN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FMN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal FMN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal FMN in samples.No significant cross-reactivity or interference between Universal FMN and analogues was observed
Storage: 2-8℃/-20℃,12 months
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