Clone Name: GT1032
Product Description: p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.
Application Note: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 18
Positive Control: HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)
Form: Liquid
Buffer (with preservative): PBS, no preservative.
Concentration: 1.45 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]
Uniprot ID: P38936
Antigen Species: Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
Purification: Affinity purified by Protein A.
Conjugation: Unconjugated
Full Name: cyclin dependent kinase inhibitor 1A
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