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Applications: Useful for labeling cells expressing PD-1 for flow cytometry and immunofluorescence microscopy.
Assay Conditions: For flow cytometry, we recommend incubating cells on ice for 30 minutes with and 1 - 20 µg/ml of RPE anti-human PD-1 antibody.
Concentration: 5.14 mg/ml
Description: R-Phycoerythrin-labeled anti-PD-1 neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1. This antibody does not cross-react with mouse PD-1, but does cross-react with monkey (M. fascicularis) PD-1. It has not been tested with other species.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C.
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1.Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.2.James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.