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Anti-Transferrin Receptor Antibody, FITC-Labeled
Anti-Transferrin Receptor Antibody, FITC-Labeled
Artikelnummer
BPS101876-1
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
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Applications: Useful for studying expression of TfR on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.
Assay Conditions: One million TFRC HEK293 cells (BPS Bioscience #78308) were blocked and stained with 1 µg of anti-TFR antibody, FITC-labeled (BPS Bioscience #101876) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. TFRC HEK293 cells (green) were compared to parental HEK293 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.
Background: Transferrin Receptor Protein-1 (also known as TfR1 or CD71) is a cell surface receptor that binds holo-transferrin (Tf) with high affinity, forming an iron-Tf-TfR complex that is endocytosed. TfR1 is crucial for cellular health, as iron acts as a co-factor for many enzymes involved in metabolism and ROS (reactive oxygen species) formation. Deregulated iron levels, and consequently ROS formation, correlate with multiple disease states including Parkinson's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Multiple Sclerosis (MS), and tumorigenesis. TfR1 is upregulated on the surface of tumor cells and endothelial cells in the brain, presenting TfR1 as a promising candidate for targeted therapy in leukemia, lymphoma and neurological diseases. Studies of TfR1 function and expression patterns will broaden our knowledge and support possible applications in therapy.
Cross Reactivity: This antibody recognizes human TfR. It has not been tested in other species.
Description: This anti-Transferrin Receptor (TfR) antibody is a purified recombinant antibody, which is labeled with Fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human TfR on the surface of Transferrin Receptor Protein-1 (TFRC) HEK293 Recombinant Cell Line (BPS Bioscience #78308).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Uniprot: P02786
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Applications: Useful for studying expression of TfR on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.
Assay Conditions: One million TFRC HEK293 cells (BPS Bioscience #78308) were blocked and stained with 1 µg of anti-TFR antibody, FITC-labeled (BPS Bioscience #101876) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. TFRC HEK293 cells (green) were compared to parental HEK293 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.
Background: Transferrin Receptor Protein-1 (also known as TfR1 or CD71) is a cell surface receptor that binds holo-transferrin (Tf) with high affinity, forming an iron-Tf-TfR complex that is endocytosed. TfR1 is crucial for cellular health, as iron acts as a co-factor for many enzymes involved in metabolism and ROS (reactive oxygen species) formation. Deregulated iron levels, and consequently ROS formation, correlate with multiple disease states including Parkinson's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Multiple Sclerosis (MS), and tumorigenesis. TfR1 is upregulated on the surface of tumor cells and endothelial cells in the brain, presenting TfR1 as a promising candidate for targeted therapy in leukemia, lymphoma and neurological diseases. Studies of TfR1 function and expression patterns will broaden our knowledge and support possible applications in therapy.
Cross Reactivity: This antibody recognizes human TfR. It has not been tested in other species.
Description: This anti-Transferrin Receptor (TfR) antibody is a purified recombinant antibody, which is labeled with Fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human TfR on the surface of Transferrin Receptor Protein-1 (TFRC) HEK293 Recombinant Cell Line (BPS Bioscience #78308).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Uniprot: P02786
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101876-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101876-1 |
| Green Labware | Nein |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Klonalität | Monoclonal |
| Isotyp | IgG1 |
| Wirt | Human |
| Produktinformation (PDF) |
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| MSDS (PDF) |
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Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
