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Application: Useful for studying expression of DLL3 on the cell surface by flow cytometry.
Assay Conditions: One million DLL3 CHO cells (#78882) were blocked and stained with 1 µg of Anti-DLL3 Antibody, APC-Labeled (#102820) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. DLL3 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis indicates the cell count. X-axis represents APC intensity.
Background: DLL3, also known as delta like ligand three, is a Notch ligand characterized by a DSL domain, transmembrane region, and a series of EGF-like (epidermal growth factor) repeats. Notch ligands can participate in trans-interactions (interaction with Notch receptor on a different cell) and cis interactions (interaction with Notch receptor within the same cell) to activate or inhibit Notch signaling, respectively. DLL3 exclusively functions to inhibit Notch signaling through cis inhibition. While DLL3 expression is limited in healthy tissue, high expression levels of DLL3 are found in various cancers including small cell lung cancer (SCLC), where it plays an oncogenic role.
Cross Reactivity: This antibody recognizes human DLL3. It has not been tested in other species.
Description: This anti-DLL3 (delta like ligand three) antibody is a purified monoclonal recombinant antibody, which is labeled with Allophycocyanin (APC). This antibody has been tested by flow cytometry for binding to human DLL3 on DLL3 CHO Cell Line (BPS Bioscience #78882). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥85%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Target: DLL3
Uniprot: Q9NYJ7
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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