Product Description: HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various physiological and pathological conditions including normal development and cancer.
Application Note: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:570. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 93
Positive Control: HepG2 (1% O2 treatment for 24hr) , 293T transfected HIF1A
Specificity/Sensitivity: Knockdown/Knockout validation was supported by references (PMID:25428606)
Form: Liquid
Buffer (with preservative): PBS, 20% Glycerol, 0.025% ProClin 300.
Concentration: 1.38 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Uniprot ID: Q16665
Antigen Species: Human
Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: hypoxia inducible factor 1 subunit alpha
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