Product Description: LC3B antibody recognizes LC3B, an autophagosomal membrane structural protein with a predicted molecular weight of 14.7 kDa. LC3B protein is a member of the highly conserved Atg8 protein family, which encompasses the LC3 and GABARAP subfamilies. The key function of LC3B involves autophagy substrate selection during autophagosome formation. Therefore, LC3B antibody is of great utility for the study of autophagosome biogenesis.
Application Note: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 15
Positive Control: Huh7 (HCV-infected) , Huh7 (3uM-Thapsigargin treatment for 12hr) , mouse brain , Rat brain , NT2D1 , PC-3 , U87-MG , SK-N-SH , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , HeLa (50μM Chloroquine treatment for 24 hr)
Specificity/Sensitivity: Cross-reactivity may occur with other LC3 isoforms
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 0.34 mg/ml (Please refer to the vial label for the specific concentration.)
Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
Uniprot ID: Q9GZQ8
Antigen Species: Human
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: microtubule associated protein 1 light chain 3 beta
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