Product Description: SQSTM1 antibody, also known as ubiquitin-binding protein p62 antibody, detects the SQSTM1 protein that interacts with LC3 proteins to execute autophagosomal degradation. SQSTM1 is also reported to regulate the activation of NFκB1 downstream signaling in addition to having other cellular functions.
Application Note: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 48
Positive Control: NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
Uniprot ID: Q13501
Antigen Species: Human
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: sequestosome 1
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