Product Description: Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.
Application Note: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 54
Positive Control: 293T
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 0.16 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Uniprot ID: P08670
Antigen Species: Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: vimentin
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