CloneID: 29C11
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with a mouse/human chimeric migis-αL-containing protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.
Application Notes (Clone): Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).
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