Application Note: Anti-Rat IgG HRP antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:10,000 - 1:40,000
Immunohistochemistry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:1,000 - 1:4,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in goat.
Physical State: Lyophilized
Purity and Specificity: Anti-Rat IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Background: Fab Anti-Rat IgG HRP conjugated secondary antibody generated in goat detects specifically rat IgG. This secondary peroxidase conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western blot, immunoprecipitation and more generally immunoassays.
Low Endotoxin: No
Secondary Antibody Specificity: IgG (H&L)
Other: User Optimized
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