Mouse anti-Human IgM antibody (SA-DA4) (FITC)

Clone Name: SA-DA4

Light Chain: Kappa

Application Note: FACS: ≦1 μg/106cells in a final volume of 100 µL. ELISA: 1:200-1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Form: Liquid

Buffer (with preservative): PBS, 0.09 % sodium azide.

Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Background: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]

Uniprot ID: P01871

Antigen Species: Human

Immunogen: Human IgM myeloma protein

Target Isotype: IgM

Purification: Purified IgG1

Conjugation: FITC

Full Name: immunoglobulin heavy constant mu

• Artikelnummer: GTX04300-06-500
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX04300-06-500
• Green Labware: Nein
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Immunohistochemistry (frozen), Immunoprecipitation, Western Blotting, ELISA, Immunocytochemistry, Fluorescence-Activated Cell Sorting, Multiplexing
• Isotyp: IgG1
• Human Gene ID: 3507
• Wirt: Mouse