Clone Name: 4A10
Light Chain: Kappa
Product Description: TSG101 antibody [4A10] is a well-validated and highly cited mouse monoclonal antibody that detects TSG101, a protein of 390 amino acids and a predicted molecular weight of 44 kDa that is a core constituent of the ESCRT-I complex. Reports have described it having both tumor suppressor and tumor enhancer activities. TSG101 is involved in exosome secretion and is commonly used as an exosome marker.
Application Note: WB: 1:500-1:3000. ICC/IF: 1:500-1:1000. IHC-P: 1:100-1:1000. FACS: 1:25-1:200. EM: . IHC: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 44
Positive Control: 293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1
Specificity/Sensitivity: Knockdown/Knockout validation was supported by references (PMID:27226592)
Form: Liquid
Buffer (with preservative): PBS, no preservative.
Concentration: 1.8 mg/ml (Please refer to the vial label for the specific concentration.)
Background: The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
Uniprot ID: Q99816
Antigen Species: Human
Immunogen: Amino acids 167-374 of TSG101 protein expressed in E. coli.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: tumor susceptibility 101
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