Product Description: 5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm[1][2][3][4].
Formula: C21H11NO5S
References: [1]J S Miller , ET AL. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry. 1990;11(6):667-75./[2]S S Twining, et al. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.Anal Biochem. 1984 Nov 15;143(1):30-4./[3]K Sugiura, et al. A simple quantitative immunofluorescence assay for FITC-labeled antibody on platelets. J Immunol Methods. 1981;40(2):165-70./[4]Natalia Oddone, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. J Nanopart Res. 2013 15:1776.
CAS Number: 27072-45-3
Molecular Weight: 389.38
Compound Purity: 97.59
Research Area: Others
Solubility: DMSO : ≥ 156 mg/mL
Target: Fluorescent Dye
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