Features: Can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays. Prepared by conjugating streptavidin to heat stable, cross-linked 4% agarose gel beads. To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm. Biotin binding activity of the streptavidin optimally preserved through proprietary coupliing procedure. Streptavidin cannot be leached from gel with solutes such as Tris buffer. Low non-specific binding activity of gel. Protein concentration is 1 mg streptavidin per ml of settled agarose beads. Supplied as a 1:1 suspension in buffer. Binding capacity is approximately 1 mole biotinylated HRP per mole streptavidin.