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Application: Screen or titrate small molecule inhibitors or antagonists that affect ATPase activity of BLM in high throughput screening (HTS) applications.
Background: The BLM helicase, also known as Bloom syndrome protein, is a key enzyme involved in DNA replication and repair (DDR). The BLM helicase is a member of the RecQ family of helicases, which are evolutionarily conserved and found in many organisms, including bacteria, yeast, and humans. It catalyzes the unwinding of duplex DNA with 3’ to 5’ directionality, driven by the energy generated from ATP hydrolysis. BLM plays a crucial role in maintaining genomic stability by unwinding DNA structures during processes such as DNA replication, recombination, and repair. Mutations in the BLM gene can lead to Bloom syndrome, a rare genetic disorder characterized by growth deficiency, sun-sensitive skin lesions, and an increased risk of cancer. High expression of BLM is found in glioblastoma, and it was found that inhibition of its activity leads to increased susceptibility to treatment with drugs targeting other proteins involved in DDR, such as PARP1 (poly-ADP ribosylation protein 1). The use of BLM inhibitors as part of a combinatory therapeutic approach may open new avenues of treatment in cancer therapy.
Contraindications: The final concentration of DMSO in the reaction should not exceed 1%.
Description: The BLM ATPase Activity Assay Kit is designed for screening and profiling of BLM (Bloom syndrome protein) antagonists/inhibitors by monitoring their effect on the conversion of ATP to ADP using ADP-Glo™ as a detection reagent. ATP conversion to ADP occurs during DNA unwinding by the ATP dependent helicase domain of BLM. BLM ATPase Activity Assay Kit comes in a convenient 96-well format, with contains enough purified recombinant BLM (amino acids 630-1300), ATP, WRN substrate, assay buffer and additives for 100 reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: BLM
Uniprot: P54132
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Chen, X., et al., 2021. eLife 10 :e65339.Wojnicki K., et al., 2023 Cell Death Discovery 9 :157.
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