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Cathepsin B Inhibitor Screening Assay Kit
Cathepsin B Inhibitor Screening Assay Kit
Artikelnummer
BPS79590-2
Verpackungseinheit
384 reactions
Hersteller
BPS Bioscience
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Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin B is a cysteine protease, of the C1 family of papain-like peptidases, that primarily functions as an endopeptidase within endo-lysosomal compartments in normal cells. Cathepsin B is closely linked to apoptosis, with activated caspase-8 leading to the release of cathepsin B to the cytosol, which in turn leads to cytochrome c release and apoptosis. It is also involved in Bcl-2 (B-cell lymphoma 2) degradation. It plays a role in inflammasome regulation, with NLRP3 (NOD and pyrin containing protein 3) activators increasing the interaction cathepsin-NLRP3 and caspase-1 activation. Cathepsin B-mediated programmed cell death (PCD) can be a cause of disease progression in abnormal tissues. High levels of cathepsin B are found in a wide variety of human cancers and in experimental models, such as transgenic models of murine pancreatic and mammary carcinomas and causal roles for this protein have been demonstrated in initiation, growth/tumor cell proliferation, angiogenesis, invasion, and metastasis. It has been proposed as a biomarker, and its levels seem to correlate with metastasis. Its multiple roles make cathepsin B an attractive target for cancer therapy.
Description: The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin B assay kit comes in a convenient 96-well format, with purified Cathepsin B, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P07858
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Xie Z., et al., 2023 Cell Death & Disease 14: 255.
Chevriaux A., et al., 2020 Front. Cell Dev. Biol. 8: 167. https://doi.org/10.3389/fcell.2020.00167.
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin B is a cysteine protease, of the C1 family of papain-like peptidases, that primarily functions as an endopeptidase within endo-lysosomal compartments in normal cells. Cathepsin B is closely linked to apoptosis, with activated caspase-8 leading to the release of cathepsin B to the cytosol, which in turn leads to cytochrome c release and apoptosis. It is also involved in Bcl-2 (B-cell lymphoma 2) degradation. It plays a role in inflammasome regulation, with NLRP3 (NOD and pyrin containing protein 3) activators increasing the interaction cathepsin-NLRP3 and caspase-1 activation. Cathepsin B-mediated programmed cell death (PCD) can be a cause of disease progression in abnormal tissues. High levels of cathepsin B are found in a wide variety of human cancers and in experimental models, such as transgenic models of murine pancreatic and mammary carcinomas and causal roles for this protein have been demonstrated in initiation, growth/tumor cell proliferation, angiogenesis, invasion, and metastasis. It has been proposed as a biomarker, and its levels seem to correlate with metastasis. Its multiple roles make cathepsin B an attractive target for cancer therapy.
Description: The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin B assay kit comes in a convenient 96-well format, with purified Cathepsin B, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P07858
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Xie Z., et al., 2023 Cell Death & Disease 14: 255.
Chevriaux A., et al., 2020 Front. Cell Dev. Biol. 8: 167. https://doi.org/10.3389/fcell.2020.00167.
| Artikelnummer | BPS79590-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 79590-2 |
| Verpackungseinheit | 384 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
