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Cathepsin L Inhibitor Screening Assay Kit
Cathepsin L Inhibitor Screening Assay Kit
Artikelnummer
BPS79591-2
Verpackungseinheit
384 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin L is a lysosomal cysteine protease that belongs to the papain-like superfamily. It is involved in many essential physiological processes, including degradation and renewal of intracellular proteins, activation of prohormones, and presentation of antigens, as well as organ development. One of its main functions is in the degradation of antigens, and it has been shown to participate in SARS-CoV-1 and SARS-CoV-2 infection, as infection requires viral membrane fusion and that seems to require proteolysis of the Spike protein by Cathepsin L. The use of amantadine inhibited Cathepsin L and prevented SARS-CoV-2 infection. Cathepsin L plays important roles in tumor metastasis and chemotherapy resistance, and upregulation of this enzyme has been reported in a wide range of human cancers including ovarian, renal, and breast carcinoma. The development of inhibitors targeting this protein may prove beneficial for both COVID-19 and cancer therapy.
Description: The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin L assay kit comes in a convenient 96-well format, with purified Cathepsin L, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P07711
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zhao M., et al., 2021 Signal Transduction and Targeted Therapy 6:134.
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin L is a lysosomal cysteine protease that belongs to the papain-like superfamily. It is involved in many essential physiological processes, including degradation and renewal of intracellular proteins, activation of prohormones, and presentation of antigens, as well as organ development. One of its main functions is in the degradation of antigens, and it has been shown to participate in SARS-CoV-1 and SARS-CoV-2 infection, as infection requires viral membrane fusion and that seems to require proteolysis of the Spike protein by Cathepsin L. The use of amantadine inhibited Cathepsin L and prevented SARS-CoV-2 infection. Cathepsin L plays important roles in tumor metastasis and chemotherapy resistance, and upregulation of this enzyme has been reported in a wide range of human cancers including ovarian, renal, and breast carcinoma. The development of inhibitors targeting this protein may prove beneficial for both COVID-19 and cancer therapy.
Description: The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin L assay kit comes in a convenient 96-well format, with purified Cathepsin L, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P07711
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zhao M., et al., 2021 Signal Transduction and Targeted Therapy 6:134.
| Artikelnummer | BPS79591-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 79591-2 |
| Verpackungseinheit | 384 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
