English
Cathepsin S Inhibitor Screening Assay Kit
Cathepsin S Inhibitor Screening Assay Kit
Artikelnummer
BPS79588-1
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin S is a cysteine protease of the C1 papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation. Contrary to other cathepsins, Cathepsin S functions at neutral pH and it is secreted in response to inflammatory triggers. Its activity is regulated by cystacin C, its endogenous inhibitor. Cathepsin S has additional biological effects, acting as one of the most potent elastases, cleaving a number of extracellular matrix (ECM) proteins. Its role as elastase contributes to blood vessel permeability and angiogenesis. High levels of this protein can be found in psoriatic keratinocytes and pulmonary fibrosis, and it has also been linked to nociception. The development of inhibitors targeting Cathepsin S can prove beneficial in the treatment of pulmonary fibrosis and other Cathepsin S-linked diseases.
Description: The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P25774
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yoo Y., et al., 2022 Biomedicine & Pharmacotherapy 145: 112245.
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin S is a cysteine protease of the C1 papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation. Contrary to other cathepsins, Cathepsin S functions at neutral pH and it is secreted in response to inflammatory triggers. Its activity is regulated by cystacin C, its endogenous inhibitor. Cathepsin S has additional biological effects, acting as one of the most potent elastases, cleaving a number of extracellular matrix (ECM) proteins. Its role as elastase contributes to blood vessel permeability and angiogenesis. High levels of this protein can be found in psoriatic keratinocytes and pulmonary fibrosis, and it has also been linked to nociception. The development of inhibitors targeting Cathepsin S can prove beneficial in the treatment of pulmonary fibrosis and other Cathepsin S-linked diseases.
Description: The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Uniprot: P25774
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yoo Y., et al., 2022 Biomedicine & Pharmacotherapy 145: 112245.
| Artikelnummer | BPS79588-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 79588-1 |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
