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Application: Isolate or deplete CD20-expressing cells from a mixed population such as PBMCs Positively selected cells, or CD20 cells (depleted population), may be used for downstream applications such as genomic analysis, expression assays, protein isolation or flow cytometry
Background: B cells are critical immune cells for functions such as antibody production, support of other mononuclear and antigen-presenting cells, and they influence inflammatory pathways. CD20 is a marker of certain B cell subsets, which include B cell blasts, B cell malignancies, pre-B cells, naÍve, mature, and activated B cells. There is also a small subset of CD20+ T cells in PBMCs. In PBMCs derived from healthy individuals, about 6-23% of the cells are CD20+ cells.
Description: The CD20 Positive Cell Isolation Kit is designed to magnetically separate CD20-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD20 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, the CD20 positive cells are immobilized along the side of the tube while CD20-negative cells remain in suspension and can be easily removed.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit provides enough reagents and materials for isolation of CD20+ cells from up to 1 x 108 or 1 x 109 PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.
Uniprot: P11836
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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