Chemi-Verse™ CLK2 Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: CDC-like kinase 2 (CLK2) is part of the dual-specificity protein kinase (DSK) family that phosphorylate serine/arginine rich (SR) proteins that are part of the spliceosomal complex and also regulate non-splicing proteins. It is involved in cell cycle progression, apoptosis, the DNA replication checkpoint, and regulation of telomere length.  Dysfunction in pathways regulated by CLK2 can result in inflammatory and neurodegenerative diseases such as Alzheimer's disease, in hyperglycemia, and in cancer. CLK2 is upregulated in breast cancer, non-small cell lung cancer (NSCLC), glioblastoma, and GSC (glioma stem-like cell). CLK2 also contributes to fatty acid oxidation and ketogenesis and is involved in fatty liver disease. Recent findings demonstrated that CLK kinases activate PTP-1B (protein-tyrosine phosphatase) family members, and this phosphatase may be an important cellular target of CLK. The use of Lorecivivint, a CLK2 inhibitor, in a clinical trial for osteoarthritis was found to be effective and safe. The development of CLK2 inhibitors and a deeper understanding of its role in the multiple pathways where it is involved will prove crucial for the treatment of CLK2-linked diseases.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ CLK2 Kinase Assay Kit is designed to measure CLK2 (CDC like kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CLK2 kinase (amino acids 137-end), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P49760

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Song M., et al., 2023 Signal Transduction and Targeted Therapy 8: 148.

• Artikelnummer: BPS82146
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82146
• Green Labware: Nein
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK