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COX1 Inhibitor Screening Assay Kit
COX1 Inhibitor Screening Assay Kit
Artikelnummer
BPS82203
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Applications: Study of enzyme kinetics and screening small molecule inhibitors for drug discovery and high-throughput screening applications
Background: COX1 (cyclooxygenase 1), also known as prostaglandin-endoperoxide synthase 1 (PTGS), is a peroxidase involved in the formation of prostaglandins by converting arachidonic acid to prostaglandin H2. COX1 is expressed constitutively in most cells and participates in tissue homeostasis. COX-1 promotes cytoprotection of the gastric mucosa by increasing the formation of mucus and bicarbonate, reducing acid and pepsin secretion and keeping appropriate blood flow. It is involved in the formation of thromboxane A2 (TxA2) in platelets, which stimulates aggregation and vasoconstriction. COX1 and COX2 differ in position 523, with COX2 having a valine and COX1 an isoleucine residue, making the development of specific inhibitors a challenge. The use of COX inhibitors or NSAIDs (non-steroidal anti-inflammatory drugs) is common to manage pain and inflammation. Classical NSAIDs, such as aspirin and ibuprofen, can result in damage to the gastrointestinal system. The use of low dose aspirin can help prevent atherothrombosis and the discovery of other compounds able to inhibit COX1 may prove beneficial as coadjuvants in cardiovascular disease therapy.
Description: The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex™ Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.
Biosafety Level: Not applicable (BSL-1)
Applications: Study of enzyme kinetics and screening small molecule inhibitors for drug discovery and high-throughput screening applications
Background: COX1 (cyclooxygenase 1), also known as prostaglandin-endoperoxide synthase 1 (PTGS), is a peroxidase involved in the formation of prostaglandins by converting arachidonic acid to prostaglandin H2. COX1 is expressed constitutively in most cells and participates in tissue homeostasis. COX-1 promotes cytoprotection of the gastric mucosa by increasing the formation of mucus and bicarbonate, reducing acid and pepsin secretion and keeping appropriate blood flow. It is involved in the formation of thromboxane A2 (TxA2) in platelets, which stimulates aggregation and vasoconstriction. COX1 and COX2 differ in position 523, with COX2 having a valine and COX1 an isoleucine residue, making the development of specific inhibitors a challenge. The use of COX inhibitors or NSAIDs (non-steroidal anti-inflammatory drugs) is common to manage pain and inflammation. Classical NSAIDs, such as aspirin and ibuprofen, can result in damage to the gastrointestinal system. The use of low dose aspirin can help prevent atherothrombosis and the discovery of other compounds able to inhibit COX1 may prove beneficial as coadjuvants in cardiovascular disease therapy.
Description: The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex™ Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme reactions.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS82203 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82203 |
| Green Labware | Nein |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
