Products from BPS Bioscience require a minimum order value above 400€
Application: Study and screen compounds that inhibit the binding of IL-5 to CD125 for drug discovery in high throughput screening (HTS) applications.
Background: IL-5 (interleukin-5) is a pro-inflammatory protein that functions as an intermediary cytokine in the TH2 (T helper 2) cells response in asthma and other eosinophilic allergic diseases. IL-5 is produced by TH2 cells and ILC2 (group 2 innate lymphoid cells) cells in response to stimulation by alarmins such as TSLP (thymic stromal lymphopoietin) or IL-33, which initiate the inflammatory cascade. IL-5 functions by promoting eosinophil maturation, activation, and migration into target tissues. It binds to the IL-5 specific cell surface receptor IL-5RA (IL-5 receptor alpha, also known as CD125) and further recruits the common beta chain receptor (CSF2RB) to initiate downstream signaling through JAK1/2 (Janus kinase 1/2) and STAT5. Activated eosinophils contribute to airway damage through degranulation, resulting in the release of pro-inflammatory cytokines and enzymatic mediators contributing to local inflammation and tissue damage. IL-5 signaling plays a critical role in allergic and eosinophilic asthma subtypes and has been an active area for drug discovery. Antibodies targeting both IL-5 ligand and IL-5 Receptor (IL-5RA) are FDA (Food and Drug Administration) approved for the treatment of severe eosinophilic asthma and other eosinophilic conditions. These antibodies function by neutralizing IL-5-mediated signaling in eosinophils. Additionally, IL-5RA targeting antibodies and CAR (chimeric antigen receptor)-T cells can induce immune-mediated cytotoxicity of IL-5RA-expressing cells, including eosinophils through ADCC (antibody-dependent cellular cytotoxicity) and CAR-T cell mediated killing respectively.
Contraindications: This kit is compatible with up to 1% final DMSO concentration.
Description: The IL-5: CD125 (IL-5RA) [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-5 (Interleukin-5) and CD125 (also known as IL-5RA) protein for screening and profiling applications. This kit comes with enough purified IL-5 (amino acids 20-134(end)) and biotinylated CD125 (amino acids 21-342), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. IL-5: CD125 (IL-5RA) [Biotinylated] Inhibitor Screening Assay Kit schematic. A 96-well plate is coated with IL-5 protein. After coating and blocking, biotinylated CD125 is added in an optimized assay buffer. Next, unbound biotinylated CD125 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of CD125 binding to IL-5.Need us to run inhibitor screens or profile your compounds against IL-5 binding to IL-5RA? Check out our Immunotherapy Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: IL-5Rα
Uniprot: IL-5:P05113; CD125: Q01344
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Schleich F., et al., 2023 Eur Respir Rev 32(168): 220193.
Deutsch
English
