Firefly Luciferase-eGFP Lentivirus (EF1A Promoter/Geneticin, Hygromycin, or Puromycin)-78741-H

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Applications: Ideal as a positive control for transduction.Optimize transduction assays and track reporter protein expression over time.Generate a stable cell line expressing Firefly Luciferase and eGFP with Geneticin, Hygromycin, or Puromycin selection.

Description: The Firefly Luciferase-eGFP Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce firefly luciferase and eGFP (Luc2-P2A-eGFP) driven by an EF1A promoter (Figure 1). The luciferase and eGFP proteins are co-expressed under the EF1A promoter in the cells, allowing greater flexibility for detection of the transduced cells.Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Geneticin).Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Hygromycin).Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Puromycin).

Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS78741-H
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78741-H
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK