Firefly Luciferase-mCherry Lentivirus (Hygromycin)

Products from BPS Bioscience require a minimum order value above 400€

Applications:

• Positive control in experiments involving transduction.
• Optimize transduction assays and track reporter protein expression over time.
• Generate cell pools or stable cell lines expressing firefly luciferase and mCherry following hygromycin or puromycin selection

Background: Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. mCherry is a monomeric red fluorescent protein derived from DsRed found in the sea anemones Discosoma. It belongs to the mFruit family of monomeric red fluorescent proteins, which are improved versions of mRFP1 (monomeric red fluorescent protein 1) in terms of brightness and photostability. The use of fluorescent proteins allows for direct visualization of transfected or transduced cells under a fluorescent microscope or analysis by flow cytometry. The use of lentiviruses to introduce both luciferase and mCherry is a convenient strategy that allows expression of the markers in almost all mammalian cells and to easily determine transduction efficiency and access cellular responses.

Description: Firefly Luciferase-mCherry Lentivirus (Hygromycin or Puromycin) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain firefly luciferase and mCherry (Luc2-P2A-mCherry) driven by an EF1a promoter, and a hygromycin or puromycin selection marker (Figure 1). Luciferase and mCherry are co-expressed under the EF1A promoter in cells, allowing for flexibility in the detection of transduced cells.Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-mCherry Lentivirus (Puromycin). This is a SIN vector.

Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-mCherry Lentivirus (Hygromycin). This is a SIN vector.

Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS78933-H
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78933-H
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK