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Application: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: G9a is a histone methyltransferase that exhibits methylation activity toward H3-K9.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9aactivity for screening and profiling applications. The key to the G9a Direct Activity AssayKit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. Withthis kit, only three simple steps are required for methyltransferase detection. First,S-adenosylmethionine is incubated with a sample containing assay bufferand methyltransferase enzyme for one hour. Next, primary antibody is added.Finally, the strips are treated with an HRP-labeled secondary antibody followed byaddition of the HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
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