Description: Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Rattus norvegicus (laboratory rat), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: CF647
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% BSA, 0.05% azide
Clone: BVD2-21C11
Immunogen: Recombinant human GM-CSF protein
Antibody Reactivity: GM-CSF
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Immunol (2015) 194: 5085-5093. (Flow, intracellular; IF)
- J Exp Med (1999) 190 (6): 875-880. (ELISA)
Entrez Gene ID: 1437
Expected AB Applications: ELISA (published for clone)/Flow (intracellular) (published for clone)/IF (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/IF (published)/ELISA (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunoprecipitation: 0.5-1 ug/500 ug protein lysate/Immunofluorescence: 0.5-1 ug/mL/Immunohistology (frozen) 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Neutralization Studies order Ab without azide/Optimal dilution for a specific application should be determined by user
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