Description: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Oryctolagus cuniculus (domestic rabbit), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: HLA-DPB1/2862R
Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
Antibody Reactivity: HLA-DP
Entrez Gene ID: 3115
Z-Antibody Applications: Flow (verified)/IF (verified)
Verified AB Applications: Flow (verified)/IF (verified)
Antibody Application Notes: ELISA: For coating use Ab at 2-4 ug/mL, order Ab without BSA; Flow cytometry: 1-2 ug/million cells; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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