Description: This MAb reacts with the HLA-DRB3 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: Bra30
Immunogen: Human REH cells (non-T, non-B leukemia cell line)
Antibody Reactivity: HLA-DRB
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Infection Immunity (2002) 70(5): 2492-2501. (functional studies)
- Microbiol Immunol (2000) 44(12): 1011-1017. (Flow)
- Transplant International (2011) 24(10), 1018-1026. (Flow, IF, WB)
Entrez Gene ID: 3123
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/WB (published)
Antibody Application Notes: Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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