Shelf life (days): 730.0
Formulation: A solution in methyl acetate
Formal Name: (2-[6-(4'-hydroxy)phenoxy-3H-xanthene-3-on-9-yl]benzoic acid
Purity: ≥98%
Formula Markup: C26H16O6
Formula Weight: 424.4
CAS Number: 359010-69-8
Notes: The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including neurodegeneration, aging, cancer, and infectious diseases.{9477} There are a number of fluorescent reagents, such as 2,7-dichlorodihydrofluorescein (DCDHF), that can be used to detect free radicals, but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2).{12088} HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence.{11242} It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals.
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