Human hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit

Usage: This ELISA kit applies to the in vitro quantitative determination of Human hs-CRP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Type: Sandwich-ELISA

Assay Time: 3 h 30 min

Detection Type: Colormetric

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human hs-CRP in samples. No significant cross-reactivity or interference between Human hs-CRP and analogues was observed

Storage: 2-8℃/-20℃,12 months

Citation Link 1: https://academic.oup.com/jid/advance-article-abstract/doi/10.1093/infdis/jiac287/6633821

Citation Link 2: https://europepmc.org/article/pmc/pmc9521405?javascript_support=no

Citation Link 3: https://link.springer.com/article/10.1186/s40798-019-0212-1

• Artikelnummer: ELSE-EL-H5134
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: E-EL-H5134-96
• Verpackungseinheit: 96T
• Mengeneinheit: PAK
• Reaktivität: Human
• Methode: ELISA