Description: This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: IG217 IG266
Immunogen: Purified human IgG
Entrez Gene ID: 3500; 3501; 3502; 3503
Z-Antibody Applications: IHC, FFPE (verified)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Flow cytometry: 1 ug/million cells/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT/Immunofluorescence: 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
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