Human Insulin ELISA Kit

Shelf Life: 6 months from date of receipt

Uniprot ID: P01308

Background: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

Assay Range: 4.69 µIU - 300 µIU

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human Insulin Pre-Coated 96-well Strip Plate, 1 each
• Human Insulin Standard vial, 2 each
• Human Insulin Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• Artikelnummer: BEYE82-019
• Hersteller: Bethyl Laboratories
• Hersteller Artikelnummer: E82-019
• Verpackungseinheit: 96 wells
• Mengeneinheit: STK
• Reaktivität: Human
• Methode: ELISA
• Human Gene ID: 3630