Description: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: CF568
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% BSA, 0.05% azide
Clone: 235-1
Immunogen: Nuclei of human myeloid leukemia biopsy cells
Antibody Reactivity: Human Nuclear Antigen
Entrez Gene ID: Not Known
Z-Antibody Applications: Flow, intracellular (verified)/IF (verified)/IHC, frozen (verified)
Verified AB Applications: Flow (intracellular) (verified)/IF (verified)/IHC (frozen) (verified)
Antibody Application Notes: Immunohistology Frozen 0.5-1.0 ug/mL/Immunocytochemistry acetone-fixed cells 0.25-0.5 ug/mL/Immunofluorescence 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Immunoprecipitation 0.5-1 ug/500 ug protein lysate/Does not react with mouse, rat or chicken; others not known/Optimal dilution for a specific application should be determined by user
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