Shelf Life: 6 months from date of receipt
Protein Ref Seq ID: NP_000585.2
Uniprot ID: P01375
Background: TNF-alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. [taken from NCBI Entrez Gene (Gene ID: 7124)].
Assay Range: 24.58 pg/ml - 6000 pg/ml
Assay Type: Sandwich ELISA
Detection Method: Biotinylated-Streptavidin-HRP
Sample Type: serum_plasma_cell_culture_supernatant
Contents:
- Human TNF alpha Pre-Coated 96-well Strip Plate, 1 each
- Human TNF alpha Standard vial, 2 each
- Human TNF alpha Detection Antibody
- Assay Diluent(s)
- Streptavidin-HRP Solution, 200µl
- TMB Substrate, 12 ml
- Stop Solution, 12 ml
- 20X Wash Buffer, 25 ml
- Sealing Tape, 2 sheets
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