Clone: 1A29
Specificity: Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
Antibody Modification: Unconjugated
Presentation: PBS, 0.09% Sodium Azide
Immunogen: Rat Ax cells (a HEV derived cell line).
Gene: ICAM-1 / CD54
Description: CD54 antibody LS-C45355 is an unconjugated mouse monoclonal antibody to rat CD54 (ICAM-1). Validated for Flow, IHC, IP and WB.
Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
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