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Encompassing Amino Acids: 42-1019(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human insulin-degrading enzyme (IDE), also known as insulysin, Abeta-degrading protease, insulin protease, and insulinase, GenBank Accession No. NM_004969, a.a. 42-1019(end) with N-terminal His-tag, expressed in a Baculovirus Sf9 cell expression system. MW = 114 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 20% glycerol, 3 mM DTT, and 105 mM imidazole
Genbank: NM_004969
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P14735
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Farris, W., et al., Proc. Natl. Acad. Sci. (USA). 2003; 100(7): 4162-4167.
2. Qiu, W.Q., et al., J. Biol. Chem. 1998; 273: 32730-37238.
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