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Encompassing Amino Acids: 26-232, 25-153(end)
Application: Study and screen compounds that inhibit the binding of IL-4RA to IL-4 for drug discovery in high throughput screening (HTS) applications.
Background: Interleukin-4 is produced mainly by a subpopulation of activated T cells (Th2) that are the biologically most active helper cells for B cells and that also secrete IL-5 and IL-6. The biological activities of IL-4 are mediated by a specific receptor. The extracellular domain of the IL-4 receptor is related to the receptors for EPO (erythropoietin), IL-6, and the beta chain of the IL-2 receptor (CD122). There are two types of receptors for IL-4. Type 1 receptor is a heterodimer consisting of IL-4Ralpha (IL-4Rα) and IL-4Rgamma (IL-4Rγc), mostly present on hematopoietic cells. The type 2 receptor is a heterodimer consisting of IL-4Ralpha and IL-13Ralpha1 (Il-13Rα1). IL-4 enhances expression of MHC (major histocompatibility class) 2 antigens on B cells. It can promote their capacity to respond to other B cell stimuli and to present antigens for T cells. Pretreatment of macrophages with IL-4 prevents the production of IL-1, TNFα (tumor necrosis factor alpha) and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-γ (interferon gamma). IL-4 plays crucial roles in inflammation, fibrosis, allergic reactions, and cancer. The therapeutic exploitation of IL-4 signaling pathways can result in advances in the treatment of auto-immune disorders.
Contraindications: This kit is compatible with up to 1% final DMSO concentration.
Description: The IL-4RA: IL-4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-4RA (interleukin 4 receptor alpha) and IL-4 protein for screening and profiling applications. This kit comes with enough purified recombinant IL-4RA (amino acids 26-232), biotinylated IL-4 (amino acids 25-153(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. IL-4RA: IL-4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-4RA protein. After coating and blocking, biotinylated IL-4 is added in an optimized assay buffer. Next, unbound biotinylated IL-4 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-4 binding to IL-4RA.Need us to run inhibitor screens or profile your compounds against IL-4RA binding to IL-4? Check out our Immunotherapy Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit comes with enough purified recombinant IL-4RA (amino acids 26-232), biotinylated IL-4 (amino acids 25-153(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.
Target: IL-4, IL-4Ra
Uniprot: P05112, P24394
Unit Definition: Assay Kit
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Keegan A., et al., 2021 Fac Rev. 10:71.
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