Clone: R001
Specificity: Human IL4R. No cross-reactivity with Mouse IL4R and Rat IL4R in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Antigen Modification: aa1-232
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human IL-4R / CD124 Protein
Gene: IL4R / CD124
Description: CD124 antibody LS-C819027 is an unconjugated rabbit monoclonal antibody to human CD124 (IL4R) (aa1-232). Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA, 1. 2 µg/ml of the Rabbit Anti-Human IL4R Monoclonal antibody will block 50% of the binding of 2 µg/ml of Human IL4 to immobilized Recombinant Human IL4R ) coated at 0.5 µg/ml (100 µL/well). At 4 ug/ml, this antibody will block >80% of the binding. Neutralization: The neutralization activity of IL4R Neutralizing Antibody is Measured by its ability to neutralize IL4 induced proliferation in the human erythroleukemia cell line (TF-1). The Neutralization titer (IC50) is typically 5-10 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL4. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: IL4R, CD124, CD124 antigen, IL-4RA, IL4R nirs variant 1, IL4RA, Interleukin 4 receptor, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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