Description: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: CF568
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% BSA, 0.05% azide
Clone: IRDN/805
Immunogen: Recombinant INS protein
Antibody Reactivity: Insulin
Entrez Gene ID: 3630
Z-Antibody Applications: IHC, FFPE (verified)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Does not react with rat; others not known/Immunohistology formalin-fixed 0.5-1 ug/mL/No special pretreatment is required for staining of formalin/paraffin tissues/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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