Description: This antibody recognizes a protein of 16-27 kDa, identified as human interferon-alpha-2 (IFN-a2). Its epitope maps between aa112-148 of IFN-a2 (total aa172). This MAb is specific for IFN-a2 and does not cross-react with interferon-alpha-1. The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-a2. Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-a2 after viral infections. The IFN-a family consists of 24 or more genes or pseudo-genes. IFN-a2 is one of the two distinct families (I and II) of human IFN-a. The alpha-interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: N39
Immunogen: Purified recombinant human IFN-alpha2
Antibody Reactivity: Interferon Alpha-2
Entrez Gene ID: 3440
Antibody Application Notes: For coating for ELISA, order Ab without BSA/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Optimal dilution and staining procedure for a specific application should be determined by user/Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
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