Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-1322 (end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD1A (20 - 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human JMJD1A (also known as KDM3A or JHDM2A), GenBank Accession No. NM_018433, a.a. 2-1322 (end), with N-terminal FLAG-tag, MW=148.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ng/µl FLAG peptide, and 10% glycerol.
Genbank: NM_018433
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q9Y4C1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Du ZM. et al., PLoS One.; 6(4) : e19137 (2011).
2. Guo X. et al., Neoplasma.; 52(2): 153-157 (2011).Application Reference(s):
A scintillation proximity assay for histone demethylases (2014)
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