Target Synonym: MIB;MKI;marker of proliferation Ki;Proliferation marker protein Ki;Antigen identified by monoclonal antibody Ki;PPP1R;MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of proliferation Ki-67;Ki67;Antigen identified by monoclonal antibody Ki-67;Antigen Ki67;Antigen KI-67;Proliferation marker protein Ki-67;Antigen identified by monoclonal Ki 67;Antigen identified by monoclonal Ki-67;MIB 1;Proliferation related Ki 67 antigen;Protein phosphatase 1 regulatory subunit 105;RP11-380J17.2
Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Immunogen: A synthetic peptide corresponding to the center region of the Human Ki67/MKI67
Buffer: 0.2 μm filtered solution in PBS
Dilution: IHC-P 1:500-1:2000;ICC/IF 1:20-1:100
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