Description: Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: KU729
Immunogen: Nuclear extract of human HL-60 cells
Antibody Reactivity: Ku-Holo
Entrez Gene ID: 2547 (p70); 7520 (p80)
Z-Antibody Applications: Flow, intracellular (verified)/IF (verified)
Verified AB Applications: Flow (intracellular) (verified)/IF (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunocytochemistry acetone-fixed cells 0.25-0.5 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Does not react with mouse, rat or chicken; others not known/Optimal dilution for a specific application should be determined by user
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