MAPKAPK2, His-tag Recombinant

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Encompassing Amino Acids: 46-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase which is regulated via direct phosphorylation by p38 MAP kinase (1). In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 has been shown to be one of the substrates of MAPKAPK2, in vivo.

Description: Recombinant human MAPKAPK2 (MAPK-activated protein kinase 2), also known as MK2, GenBank Accession #NM_032960, a.a. 46-end, with an N-terminal His-tag, MW = 41 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Genbank: NM_032960

Host Cell Line: Sf9 cells

Purity: ≥85%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P49137

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Stokoe, D. et al . Biochem. J. 296: 843-849, 1993.
2. Hagood JS, Olman MA. Am. J. Respir. Cell Mol. Biol. 37(5):503-6 (2007).
3. Kotlyarov A, Gaestel M. Biochem. Soc. Trans. 30(Pt 6):959-63 (2002).

• Artikelnummer: BPS40116
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40116
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect